Temperature Effects on the Rate of Reaction of Plasma Albumin with Formaldehyde in Water Solution and Ethanol-Water-Mixtures

The effects of temperature on the rate of reaction of plasma albumin and formaldehyde in water solution and in ethanol water mixtures (1-25%) were investigated under seven temperatures from 20-45 C. The effects of the temperature variations on the reaction of plasma albumin with formaldehyde were then established from a plot of ln(At-A∞) against time at each of the varied temperatures. Also the Erying plots, logk2/T vs. 1/T as a function of different temperatures were plotted and the thermodynamic parameters were calculated from the relation ln(k2 / T) = ΔH҂/RT + ΔS҂/ R + 23.8 while the activation energy was calculated by using the expression Ea = ΔH҂ + RT and the plot of lnk against 1/T according to the Arrhenius equation. The result of the temperature dependent study in water solution showed a mean second order rate constant k2=, 2.47 (±0.1) mol s, while that in ethanol-water-mixtures showed a mean value of (2.47 (±0.4). The rate constants of the reaction in both media were observed to have decreased steadily with increase in temperatures, it could then be asserted that the reaction was not favoured at high temperatures in both media.